ParkinsonCanadaV1, Main, Exploration, bibRecord, 003666

Expression of dopamine receptors in the subthalamic nucleus of the rat: characterization using reverse transcriptase–polymerase chain reaction and autoradiography

Identifieur interne : 003666 ( Main/Exploration ); précédent : 003665; suivant : 003667

Expression of dopamine receptors in the subthalamic nucleus of the rat: characterization using reverse transcriptase–polymerase chain reaction and autoradiography

Auteurs : G. Flores [Mexique] ; J. J Liang ; A. Sierra [Mexique] ; D. Mart Nez-Fong [Mexique] ; R. Quirion ; J. Aceves [Mexique] ; L. K Srivastava

Source :

Neuroscience [ 0306-4522 ] ; 1999.

RBID : ISTEX:CA5D9D1CBE83B5A440401974B0297537B81FC221

Descripteurs français

Pascal (Inist)
- Animal, Autoradiographie, Caractérisation, Dopamine, Expression génique, Locus niger, Noyau gris central, Noyau sousthalamique, Parkinson maladie, Physiopathologie, Protéine transport, Rat, Réaction chaîne polymérase, Récepteur dopaminergique.

English descriptors

KwdEn :
- Animal, Autoradiography, Basal ganglion, Carrier protein, Characterization, Dopamine, Dopamine receptor, Gene expression, Locus niger, Parkinson disease, Pathophysiology, Polymerase chain reaction, Rat, Subthalamic nucleus.

Abstract

We analysed the expression of dopamine receptor subtypes in the subthalamic nucleus by means of reverse transcriptase–polymerase chain reaction. We also studied, using autoradiography, all pharmacologically characterized dopamine receptors in four subregions of the subthalamic nucleus. For comparison, dopamine receptor subtypes were also evaluated in brain regions where they are more abundant and well characterized. The radioligands used were: [3H]SCH-23390, [3H]emonapride and [3H]2-dipropylamino-7-hydroxy-1,2,3,4-tetrahydronaphthalene for dopamine D1, D2 and D3 receptors, respectively; and [3H]YM-09151-2 in the presence of raclopride for dopamine D4 receptors. Finally, we also evaluated the effect of unilateral 6-hydroxydopamine injection into the medial forebrain bundle on dopamine receptor levels expressed in the ipsilateral subthalamic nucleus. The lesion was estimated by decrease in the binding of [3H]WIN-35428, a specific dopamine transporter label. D1, D2 and D3 receptor messenger RNAs and binding sites were present in the subthalamic nucleus, but no messenger RNA for D4 receptors was found, although specific binding sites for these receptors were observed. As compared to the intact side, the 6-hydroxydopamine lesion did not change D1 receptors, increased D2 receptors, and decreased D3 receptors and the dopamine transporter. The results suggest that postsynaptic D1, D2 or D3 receptors can mediate the effect of dopamine on subthalamic nucleus neuronal activity. D4 receptors would mediate exclusively presynaptic effects. These results reinforce the idea that dopamine receptors in the subthalamic nucleus may play an important role in the physiology of the basal ganglia and in the pathophysiology of Parkinson's disease.

Url:

https://api-v5.istex.fr/document/CA5D9D1CBE83B5A440401974B0297537B81FC221/fulltext/pdf

DOI: 10.1016/S0306-4522(98)00633-2

Affiliations:

Mexique

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La maladie de Parkinson au Canada (serveur d'exploration)

Expression of dopamine receptors in the subthalamic nucleus of the rat: characterization using reverse transcriptase–polymerase chain reaction and autoradiography

Expression of dopamine receptors in the subthalamic nucleus of the rat: characterization using reverse transcriptase–polymerase chain reaction and autoradiography

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	La maladie de Parkinson au Canada (serveur d'exploration)
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